Sphingosine kinase 2 promotes colorectal cancer cell proliferation and invasion by enhancing MYC expression

• DOI: 10.1007/s13277-015-4700-8
• Publication Date: 2016-01-05T12:44:38Z
• Authors: Lan Zhang; Xuezheng Liu; Zhongfu Zuo; Chunyan Hao; Yanmei Ma
• Keywords: 0303 health sciences; Blotting, Western; Real-Time Polymerase Chain Reaction; Immunohistochemistry; 3. Good health; Gene Expression Regulation, Neoplastic; Proto-Oncogene Proteins c-myc; Phosphotransferases (Alcohol Group Acceptor); 03 medical and health sciences; Cell Movement; Cell Line, Tumor; Humans; Neoplasm Invasiveness; Molecular Targeted Therapy; RNA, Small Interfering; Colorectal Neoplasms; Cell Proliferation

Colorectal cancer (CRC) is one of the most commonly diagnosed cancers and causes of cancer death in developed countries. SphK2 is overexpressed in a number of aggressive human carcinomas; however, the expression profile and potential function of SphK2 in CRC are still unknown. In this study, we investigated the SphK2 expression in tumoral tissue and the matched normal mucosae using quantitative real-time PCR (qRT-PCR), Western blot, and immunohistochemistry. We also evaluated the impact of SphK2 knockdown on CRC cell proliferation and metastasis in vitro. SphK2 was significantly upregulated in CRC tissue as compared to the matched normal mucosae, and significant overexpression was found in the LoVo CRC cell line. SphK2 depletion by specific small interfering RNA (siRNA) in the CRC cell line was found to affect cell proliferation and cell migration. Our data suggest that the pathogenesis of CRC maybe mediated by SphK2, and SphK2 could represent a selective target for the molecularly targeted treatments of CRC.