Piperlongumine induces gastric cancer cell apoptosis and G2/M cell cycle arrest both in vitro and in vivo

• DOI: 10.1007/s13277-016-4792-9
• Publication Date: 2016-02-13T10:19:31Z
• Authors: Chaoqin Duan; Bin Zhang; Chao Deng; Yu Cao; Fan Zhou; Longyun Wu; Min Chen; Shanshan Shen; Guifang Xu; Shu Zhang; Guihua Duan; Hongli Yan; Xiaoping Zou
• Keywords: Membrane Potential, Mitochondrial; STAT3 Transcription Factor; 0301 basic medicine; Mice, Nude; Nuclear Proteins; Apoptosis; Cell Cycle Proteins; Dioxolanes; Endoplasmic Reticulum Stress; Antineoplastic Agents, Phytogenic; Glutathione; Acetylcysteine; Neoplasm Proteins; 3. Good health; G2 Phase Cell Cycle Checkpoints; Mice; 03 medical and health sciences; Cell Line, Tumor; Animals; Humans; RNA Interference; RNA, Small Interfering; Cell Division

Recently, several studies have shown that piperlongumine (PL) can selectively kill cancer cells by targeting reactive oxygen species (ROS). However, the potential therapeutic effects and detailed mechanism of PL in gastric are still not clear. In current report, we found significantly suppressed both vitro vivo. obviously increased ROS generation cells. Anti-oxidant glutathione (GSH) N-acetyl-l-cysteine (NAC) abrogate PL-induced cell death proliferation inhibition. GADD45α was induced PL-treated led to G2/M phase arrest, whereas genetic depletion small interfering RNAs (siRNAs) could partly reverse cycle arrest Interestingly, also treatment decreased expression telomerase transcriptase (TERT) gene, which plays an essential role initiation progression. Our findings thus revealed a anti-tumor effect on may implications.