Manganese-superoxide dismutase (Mn-SOD) overexpression is a common event in colorectal cancers with mitochondrial microsatellite instability

• DOI: 10.1007/s13277-016-4918-0
• Publication Date: 2016-02-04T10:00:28Z
• Authors: Suresh Govatati; Sravanthi Malempati; Bulle Saradamma; Dasi Divyamaanasa; B. Prathap Naidu; Pallaval Veera Br...; Nagesh Narayana; Sisinthy Shivaji; Manjula Bhanoori; Raghava Rao Tamanam; Pasupuleti Sreeni...; Varadacharyulu Na...
• Keywords: Microsatellite Instability

Mitochondrial displacement loop (D-loop) is a hot spot for mitochondrial DNA (mtDNA) alterations that effects cellular reactive oxygen species (ROS) generation. Manganese-superoxide dismutase (Mn-SOD) major antioxidant enzyme protects cells from ROS-mediated damage. In the present study, we investigated relationship between sequence of D-loop and Mn-SOD expression in colorectal cancer (CRC). Genotyping entire (1124 bp) was carried out on mtDNA analogous tumor normal tissues 35 CRC patients south Indian origin by PCR-sequencing analysis. Tumor-specific large-scale deletions analyzed PCR Western blot analysis, respectively. We identified 87 polymorphisms region and/or control tissues. Polymorphisms were predominantly located hypervariable I (67.9 %) than II (32.1 D-loop. Significantly increased microsatellite instability (mtMSI) [310'C' insertion (P = 0.00001) T16189C 0.0007)] elevated observed compared with controls. Interestingly, mtMSI significantly high tumors overexpression. not conclusion, overexpression are common event CRC. The analysis might help to identify at risk disease outcome, thereby helping refine therapeutic decisions