MicroRNA-130b promotes proliferation and EMT-induced metastasis via PTEN/p-AKT/HIF-1α signaling

• DOI: 10.1007/s13277-016-4919-z
• Publication Date: 2016-02-09T21:50:48Z
• Authors: Rui-Min Chang; Jiang-Feng Xu; Feng Fang; Hao Yang; Lian-Yue Yang
• Keywords: Adult; Male; 0301 basic medicine; 2. Zero hunger; Mice, Inbred BALB C; 0303 health sciences; Carcinoma, Hepatocellular; Epithelial-Mesenchymal Transition; Liver Neoplasms; Mice, Nude; Kaplan-Meier Estimate; Middle Aged; Hypoxia-Inducible Factor 1, alpha Subunit; Disease-Free Survival; 3. Good health; Gene Expression Regulation, Neoplastic; Mice; MicroRNAs; 03 medical and health sciences; Cell Line, Tumor; Animals; Heterografts; Humans; Female; Aged

Hepatocellular carcinoma (HCC) is a major cause of cancer-related deaths owing to its high rate postoperative recurrence and metastasis. New research continuously identifying novel metastasis-associated oncogenes tumor suppressor genes. miRNAs are noncoding RNAs that regulate protein synthesis post-translationally. miR-130b one several involved in However, the role HCC remains controversial. Here, we demonstrate highly expressed it correlates with number, vascular invasion, TNM stage—important predictors metastases. Moreover, levels predicted poor overall disease-free survival patients, vitro vivo revealed knockdown or overexpression inhibited promoted proliferation metastasis cells, respectively. We identified PTEN as direct functional target using miRNA databases dual luciferase report assay. Next, gain loss assay epithelial-mesenchymal transition (EMT) relative assays, show may promote EMT-induced via PTEN/p-AKT/HIF-1α signaling. Collectively, our data suggests have prognostic value HCC. Additionally, miR-130b/PTEN/p-AKT/HIF-1α axis this study provides insight into mechanisms metastasis, which facilitate development new therapeutics against