DDR1 enhances invasion and metastasis of gastric cancer via epithelial-mesenchymal transition

• DOI: 10.1007/s13277-016-5070-6
• Publication Date: 2016-05-14T05:53:23Z
• Authors: Ruixia Xie; Xiaoying Wang; Guoqing Qi; Zhiping Wu; Rong Wei; Peirong Li; Dekui Zhang
• Keywords: DDR1

In this study, we investigated the effects of DDR1 on invasion and metastasis in gastric cancer (GC) via epithelial-mesenchymal transition (EMT). Immunohistochemistry analysis was used to detect DDR1, E-cadherin, Vimentin expression GC tissues as well cell lines normal epithelial cells. The relationship between EMT explored by down upregulating examining corresponding changes EMT-related proteins biological characteristics. Furthermore, a nude mice model with transplantation tumor generating from stably transfected cells overexpression established performed further reveal cellular morphology growth GC. Our results showed that highly expressed compared adjacent line, its significantly higher having poor differentiation (p < 0.01), advanced depth wall = 0.020), lymph node 0.0001), liver high TNM stage 0.01). Western blot analyses revealed resulted significant decrease E-cadherin 0.01) an increase Snail while knockdown led opposite outcomes. We demonstrated promoted proliferation 0.05), migration accelerated 0.05) microvessel formation mice. study establishes enhances EMT.