Effects of Olig2-Overexpressing Neural Stem Cells and Myelin Basic Protein-Activated T Cells on Recovery from Spinal Cord Injury

• DOI: 10.1007/s13311-011-0090-9
• Publication Date: 2011-12-15T13:49:45Z
• Authors: Jian-Guo Hu; Lin Shen; Rui Wang; Qi-Yi Wang; Chen Zhang; Jin Xi; Shan-Feng Ma; Jian-Sheng Zhou; He-Zuo Lü
• Keywords: OLIG2

Neural stem cell (NSC) transplantation is a major focus of current research for treatment spinal cord injury (SCI). However, it very important to promote the survival and differentiation NSCs into myelinating oligodendrocytes (OLs). In this study, myelin basic protein-activated T (MBP-T) cells were passively immunized improve SCI microenvironment. Olig2-overexpressing infected with lentivirus carrying enhanced green fluorescent protein (GFP) reporter gene generate Olig2-GFP-NSCs that transplanted injured site differentiate OLs. Transferred MBP-T infiltrated cord, produced neurotrophic factors, induced resident microglia and/or infiltrating blood monocytes an "alternatively activated" anti-inflammatory macrophage phenotype by producing interleukin-13. As result, increased fivefold in cell-transferred rats compared vehicle-treated control. addition, MBP-positive OLs 12-fold Olig2-GFP-NSC-transplanted GFP-NSC-transplanted controls. Olig2-GFP-NSC combined group, number OL-remyelinated axons significantly those all other groups. significant decrease lesion volume increase spared behavioral recovery observed Olig2-NSC- NSC-transplanted Collectively, these results suggest adoptive immunotherapy NSC has synergistic effect on histological improvement after traumatic SCI. Although Olig2 overexpression enhances OL myelination, functional may be surpassed cells.