Structural characteristics of ScBx genes controlling the biosynthesis of hydroxamic acids in rye (Secale cereale L.)

• DOI: 10.1007/s13353-015-0271-z
• Publication Date: 2015-02-09T11:20:29Z
• Authors: Beata Bakera; Bogna Makowska; Jolanta Groszyk; Michał Niziołek; Wacław Orczyk; Hanna Bolibok-Brą...; Aneta Hromada-Jud...; Monika Rakoczy-Tr...
• Keywords: Secale; Hydroxamic acid

Benzoxazinoids (BX) are major secondary metabolites of gramineous plants that play an important role in disease resistance and allelopathy. They also have many other unique properties including anti-bacterial anti-fungal activity, the ability to reduce alfa-amylase activity. The biosynthesis modification BX controlled by genes Bx1 ÷ Bx10, GT glu, majority these Bx been mapped maize, wheat rye. However, genetic basis remains largely uncharacterized apart from some data maize wheat. aim this study was isolate, sequence characterize five (ScBx1, ScBx2, ScBx3, ScBx4 ScBx5) encoding enzymes involved synthesis DIBOA, defense compound Using a modified 3D procedure BAC library screening, seven clones containing all ScBx were isolated sequenced. Bioinformatic analyses resulting contigs used examine structure features genes, their promoters, introns 3'UTRs. Comparative analysis showed similar those Poaceae species, especially TaBx genes. polymorphisms present both coding sequences non-coding regions relation predicted impact on expression, encoded proteins.