Laser Desorption/Ionization Mass Spectrometric Imaging of Endogenous Lipids from Rat Brain Tissue Implanted with Silver Nanoparticles

• DOI: 10.1007/s13361-017-1665-4
• Publication Date: 2017-04-21T14:44:43Z
• Authors: Ludovic Muller; Kathrine Baldwin; Damon C. Barbacci; Shelley N. Jackson; Aurélie Roux; Carey D. Balaban; Bruce E. Brinson; Michael I. McCully; Ernest K. Lewis; J. Albert Schultz; Amina S. Woods
• Keywords: Mass spectrometry imaging

Mass spectrometry imaging (MSI) of tissue implanted with silver nanoparticulate (AgNP) matrix generates reproducible lipids in rodent models disease and injury. Gas-phase production acceleration size-selected 8 nm AgNP is followed by controlled ion beam rastering soft landing implantation 500 eV into tissue. Focused 337 laser desorption produces high quality images for most lipid classes rat brain (in positive mode: galactoceramides, diacylglycerols, ceramides, phosphatidylcholines, cholesteryl ester, cholesterol, negative phosphatidylethanolamides, sulfatides, phosphatidylinositol, sphingomyelins). Image reproducibility serial sections achieved within <10% tolerance selecting argentated instead alkali cationized ions. The tissues spotted pure standards was used to demonstrate that Ag ceramide diacylglycerol ions are from intact, endogenous species. In contrast, almost all fatty acid a result fragmentations numerous types having the as subunit. Almost no intact come standard on Graphical Abstract ᅟ.