Decreasing Carbon Tetrachloride Toxicity using Date-seed (Phoenix dactylifera L.) Steeping in Rats

• DOI: 10.1007/s13530-018-0357-1
• Publication Date: 2018-07-11T10:46:02Z
• Authors: Saryono Saryono; Annas Sumeru; Atikah Proverawati; Ferry Efendi
• Keywords: 0303 health sciences; 03 medical and health sciences; 3. Good health

Objective: Many toxic compounds in foods cause liverdamage and disturbance of bodily function. Inflammationwill precede liver damage as an initial response topoisoning. The inflammatory response depends heavilyon the strength of the body’s immune system. Manyfoods, drugs, and other compounds can decrease theimmune system, but few serve as immunostimulants.This study aims to prove the decreasing of carbon tetrachloridetoxicity using date-seed (Phoenix dactyliferaL.) steeping to improve rat immunity.Methods: This was an experiment with pre- and posttestwith a control group design. Wistar white rats weregrouped into 6 groups, healthy control (HC), negativecontrol (NC), positive control (PC), treatment dose 1 g/kg (T1), treatment dose 3 g/kg (T3), and treatment dose5 g/kg (T5). All of the groups were induced by CCL4before treatment except the HC group. The observeddata were interleukin-6 (IL-6), lymphocyte count, andC-reactive protein (CRP). Data from the groups werecompared with an ANOVA test and followed by a posthoc test if a significant result was found.Results: The results showed that there were significantdifferences between IL-6, lymphocyte count, andCRP between HC and other groups that CCL4-induced.After the delivery of date-seed steeping, levelsof IL-6 and CRP decreased, and the lymphocytecount increased significantly. The group with the 5 g/kg treatment dose was the most effective group forinhibiting the increase of IL-6 and CRP, but a dose of 3g/kg was the most effective to increase lymphocytecount.Conclusion: Date-seed steeping suppresses pro-inflammationmediators; it has a potency which improvescytokine pro-inflammation by inhibiting the inflammationprocess. Thus, date seed can be used as a cheapsource of anti-inflammation that can be considered asa health opportunity for developing countries.