Cancer cachexia is associated with a decrease in skeletal muscle mitochondrial oxidative capacities without alteration of ATP production efficiency

• DOI: 10.1007/s13539-012-0071-9
• Publication Date: 2012-05-30T09:07:33Z
• Authors: Cloé M. Julienne; Jean‐François Dumas; Caroline Goupille; Michelle Pinault; Cécile Berri; Anne Collin; Sophie Tesseraud; Charles Couet; Stephane Servais
• Keywords: Bioenergetics; Anaerobic glycolysis

Cancer cachexia is a complex syndrome related to negative energy balance resulting in muscle wasting. Implication of mitochondrial bioenergetics alterations during cancer was suggested. Therefore, the aim this study explore efficiency oxidative phosphorylation skeletal mitochondria preclinical model cachexia.Berlin-Druckrey IX rats with peritoneal carcinosis (PC) were used as healthy pair-fed (PF) control. Hindlimb morphology and fibre type composition analysed parallel ubiquitin ligases UCP gene expression. Oxidative investigated isolated by measuring oxygen consumption ATP synthesis rate.PC underwent significant wasting affecting fast glycolytic muscles due reduction cross-sectional area. MuRF1 MAFbx expression significantly increased (9- 3.5-fold, respectively) PC compared PF rats. Oxygen non-phosphorylating state ATP/O similar both groups. Muscle UCP2 overexpressed State III uncoupled lower from IV activity (-30 %).This demonstrated that there neither alteration nor uncoupling cachectic despite overexpression. capacities reduced decrease activity. This could participate insulin resistance, lipid droplet accumulation lactate production.