Establishment and characterization of NCC-DDLPS3-C1: a novel patient-derived cell line of dedifferentiated liposarcoma
Chromosomes, Human, Pair 12
Carcinogenesis
Gene Amplification
Cyclin-Dependent Kinase 4
Mice, Nude
Antineoplastic Agents
Proto-Oncogene Proteins c-mdm2
Liposarcoma
Middle Aged
Prognosis
3. Good health
Mice
03 medical and health sciences
0302 clinical medicine
Cell Line, Tumor
Spheroids, Cellular
Animals
Humans
Female
Neoplasm Invasiveness
Drug Screening Assays, Antitumor
Cell Proliferation
DOI:
10.1007/s13577-021-00515-1
Publication Date:
2021-03-06T18:03:01Z
AUTHORS (12)
ABSTRACT
Dedifferentiated liposarcoma (DDLPS) is a highly malignant subtype of liposarcoma, with characteristic amplification of MDM2 and CDK4 (12q14-15). It is caused by the dedifferentiation of well-differentiated liposarcoma. DDLPS is refractory to conventional chemotherapy; thus, surgical resection is the primary treatment modality. However, complete resection of DDLPS is difficult because of its deep location, which results in poor prognosis. Therefore, novel systemic chemotherapy is required to improve the clinical outcome. Patient-derived cell lines are important tools in the development of novel chemotherapy. However, there are no DDLPS cell lines available from public cell banks. In this study, we established a novel DDLPS cell line, NCC-DDLPS3-C1, using a surgically resected specimen from a patient with DDLPS. NCC-DDLPS3-C1 cells retained the characteristic gene amplification of MDM2 and CDK4. In addition, other gene amplifications and losses related to the poor prognosis of DDLPS were also observed in concordance with the original tumor. The cells also exhibited rapid cell proliferation, aggressive invasion ability, spheroid formation ability, and tumorigenic ability in nude mice. Furthermore, a drug-screening test showed significant antiproliferative effects of proteasome inhibitors and HDAC inhibitors. Thus, the NCC-DDLPS3-C1 cell line should be a useful tool for the development of novel chemotherapy for DDLPS.
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