Establishment and characterization of NCC-MFS4-C1: a novel patient-derived cell line of myxofibrosarcoma

• DOI: 10.1007/s13577-021-00589-x
• Publication Date: 2021-08-12T10:03:36Z
• Authors: Yuki Yoshimatsu; Rei Noguchi; Ryuto Tsuchiya; Yooksil Sin; Takuya Ono; Jun Sugaya; Shintaro Iwata; Akihiko Yoshida; Akira Kawai; Tadashi Kondo
• Keywords: Myxofibrosarcoma; Romidepsin

Myxofibrosarcoma (MFS) is an aggressive sarcoma with a highly complex karyotype. Complete resection is the only curative treatment for MFS because it is refractory to chemotherapy. To improve clinical outcomes, it is critical to develop novel treatments for MFS. Although patient-derived cell lines play a key role in cancer research, only 12 MFS cell lines have been reported to date, and considering the diversity of the disease, more cell lines need to be established. Hence, in the present study, we established a novel MFS cell line, NCC-MFS4-C1, using a surgically resected tumor tissue from a patient with MFS. NCC-MFS4-C1 cells exhibited copy number alterations similar to those of the original tumors and showed constant proliferation, spheroid formation, and aggressive invasion. By screening a drug library, we found that actinomycin D, bortezomib, docetaxel, eribulin, and romidepsin significantly reduced the proliferation of NCC-MFS4-C1 cells. Therefore, the NCC-MFS4-C1 cell line may be a useful resource for researching MFS.