Insights into the characteristics of primary radioresistant cervical cancer using single-cell transcriptomics

• DOI: 10.1007/s13577-023-00882-x
• Publication Date: 2023-03-03T14:02:56Z
• Authors: Biyuan Xing; Congli Pu; Yunshang Chen; Yuhan Sheng; Baofang Zhang; Jie Cui; Gang Wu; Yingchao Zhao
• Keywords: Radioresistance; Radiosensitivity; clone (Java method)

Abstract Radioresistance is a major cause of radiotherapy failure among patients with cervical cancer (CC), the fourth most common mortality in women worldwide. Traditional CC cell lines lose intra-tumoral heterogeneity, posing challenge for radioresistance research. Meanwhile, conditional reprogramming (CR) maintains heterogeneity and complexity, as well genomic clinical characteristics original cells tissues. Three radioresistant two radiosensitive primary were developed under CR conditions from patient specimens, their verified via immunofluorescence, growth kinetics, clone forming assay, xenografting, immunohistochemistry. The had homogenous tumor tissues maintained radiosensitivity vitro vivo, while also maintaining according to single-cell RNA sequencing analysis. Upon further investigation, 20.83% aggregated G2/M cycle phase, which sensitive radiation, compared 38.1% lines. This study established three through CR, will benefit research investigating CC. Our present may provide an ideal model on development potential therapeutic targets