Antiproliferative polyketides from fungus Xylaria cf. Longipes SWUF08-81 in different culture media

• DOI: 10.1007/s13659-023-00427-7
• Publication Date: 2024-01-06T00:08:37Z
• Authors: Kittiwan Sresuksai; Sasiphimol Sawads...; Phongphan Jantaharn; Pakin Noppawan; Audomsak Churat; Nuttika Suwannasai; Wiyada Mongkoltha...; Thanaset Senawong; Sarawut Tontapha; Pairot Moontragoon; Vittaya Amornkitb...; Sirirath McCloskey
• Keywords: HeLa; Dibenzofuran; Cancer cell lines; MTT assay; Bioorganic chemistry

Abstract Bioactive compounds from the wood-decay fungus Xylaria cf. longipes SWUF08-81, cultivated in three different culture media (GM, YM and PDB), were isolated. Their structures stereochemistry deduced spectroscopic MS data analysis, together with quantum chemical calculations of 13 C NMR shifts electronic circular dichroism (ECD) spectra. Five undescribed polyketides including dibenzofuran ( 1 ), mellein 2 dihydroisocoumarin 15 two pyrans 16 , 17 twenty-three determined. Compounds 18 20 significantly toxic against cancer cell lines (HCT116, HT29, MCF-7 HeLa) based on MTT assay. Quantification by HPLC showed that was produced three-fold higher broth PDB than YM. These studies production primarily dependent nutrition sources it has given a starting point for growth optimization conditions scaling up bioactive production. Graphical