Alterations of DNA methylation profile in peripheral blood of children with simple obesity

• DOI: 10.1007/s13755-024-00275-w
• Publication Date: 2024-03-18T07:01:57Z
• Authors: Yi Ren; Peng Huang; Xiaoyan Huang; Lu Zhang; Lingjuan Liu; Wei Xiang; Liqun Liu; Xiaojie He
• Keywords: CpG site; Differentially methylated regions

Abstract Purpose To investigate the association between DNA methylation and childhood simple obesity. Methods Genome-wide analysis of was conducted on peripheral blood samples from 41 children with obesity 31 normal controls to identify differentially methylated sites (DMS). Subsequently, gene functional genes (DMGs) carried out. After screening characteristic DMGs based specific conditions, levels these DMS were evaluated verified by pyrosequencing. Receiver operating (ROC) curve assessed predictive efficacy corresponding DMGs. Finally, Pearson correlation revealed clinical data. Results The overall level in group significantly lower than normal. A total 241 identified. Functional pathway that primarily involved lipid metabolism, carbohydrate amino acid human diseases, among other pathways. within Transcription factor mitochondrial ( TFAM ) Piezo type mechanosensitive ion channel component 1( PIEZO1 recognized as CpG-cg05831083 CpG-cg14926485, respectively. Furthermore, correlated body mass index (BMI) vitamin D. Conclusions Abnormal is closely related altered CpG-cg14926485 could potentially serve biomarkers for