Comparison of Biological Characteristics of Human Umbilical Cord Wharton’s Jelly-Derived Mesenchymal Stem Cells from Extremely Preterm and Term Infants

• DOI: 10.1007/s13770-023-00538-9
• Publication Date: 2023-05-30T15:03:04Z
• Authors: Peng Huang; Xiaofei Qin; Chuiqin Fan; Manna Wang; Fuyi Chen; Maochuan Liao; Huifeng Zhong; Hongwu Wang; Lian Ma
• Keywords: Wharton's jelly; CD90; Cord blood

Abstract Background: Despite the progress in perinatal-neonatal medicine, complications of extremely preterm infants continue to constitute major adverse outcomes neonatal intensive care unit. Human umbilical cord Wharton’s Jelly-derived mesenchymal stem cells (HUMSCs) may offer new hope for treatment intractable disorders. This study will explore functional differences HUMSCs between and term infants. Methods: UMSCs from 5 infants(weeks gestation: 22 +5 w,24 +4 w,25 +3 w,26 w,28 w) 2 infants(39 w,39 +2 were isolated, markers, pluripotent genes, proliferation rate analyzed. HUVECs injured by treated with LPS repaired co-cultured different gestational ages. Results: All showed fibroblast-like adherence plastic positively expressed surface marker CD105,CD73 CD90, but did not CD45,CD34,CD14,CD79a HLA-DR; exhibited significant increase as evidenced CCK8, pluripotency markers OCT-4 tested RT-PCR also increase. Above all, induced inflame systerm, more capable promote wound healing tube formation HUVEC cultures, they promoted TGFβ1 expression inhibited IL6 expression. Conclusions: Our results suggest that be suitable candidates cell therapy