Cytotoxicity and Oxidative Stress Alterations Induced by Aldrin in BALB/c 3T3 Fibroblast Cells

• DOI: 10.1007/s40011-015-0694-7
• Publication Date: 2016-01-08T12:34:00Z
• Authors: M. K. Lonare; Bhaskar Vemu; A. K. Singh; V. K. Dumka; Saloni Singla; S. K. Sharma
• Keywords: Aldrin

The intensive use of pesticides in agro-vet practices resulted in their persistence in environmental medium responsible for adverse effect on living system. The present study was planned to evaluate the cytotoxicity and oxidative stress potential of aldrin on BALB/c 3T3 mouse fibroblast cells. Short exposure of 3 h was given to the BALB/c 3T3 fibroblast cells under standard in vitro conditions. Inhibitory concentration-50 was estimated at the end of exposure period by neutral red uptake assay and was found to be 49.7 μg/ml. Further, cells were exposed with three concentrations of aldrin (12.4, 24.8 and 49.7 μg/ml) and 0.1 % dimethyl sulfoxide was used as negative control. Lipid peroxidation, antioxidant enzymes and non-enzymes were determined along with glucose-6-phosphate dehydrogenase, lactate dehydrogenase and alkaline phosphatase enzymes in BALB/c 3T3 fibroblast cells. Cells were also monitored for cell morphology during experiment. Exposure of aldrin to BALB/c 3T3 fibroblast cells resulted in increase in lipid peroxidation and decrease in antioxidant enzyme/non-enzyme system. Further, it caused decrease in glucose-6-phosphate dehydrogenase enzyme activity and excess leakage of lactate dehydrogenase and alkaline phosphatase enzymes into the medium. Aldrin-treated cells showed high degree of alteration in cell morphology indicating cell damage and death. These changes were noticed in dose dependent manner. In conclusion, the result of present study suggests that increase in lipid peroxidation and decrease in activity of antioxidant defence via oxidative stress by aldrin in BALB/c 3T3 cells were responsible for cytotoxicity.