Differential methylation analysis for bisulfite sequencing using DSS

• DOI: 10.1007/s40484-019-0183-8
• Publication Date: 2019-12-07T10:02:31Z
• Authors: Hao Feng; Hao Wu
• Keywords: Bioconductor; Bisulfite sequencing; R package

Bisulfite sequencing (BS‐seq) technology measures DNA methylation at single nucleotide resolution. A key task in BS‐seq data analysis is to identify differentially (DM) under different conditions. Here we provide a tutorial for DM using Bioconductor package DSS. DSS uses beta‐binomial model characterize the sequence counts from BS‐seq, and implements rigorous statistical method hypothesis testing. It provides flexible functionalities variety of analyses.