Characterization of dendritic cell subtypes in human cord blood by single-cell sequencing

• DOI: 10.1007/s41048-019-00096-5
• Publication Date: 2019-10-01T01:59:50Z
• Authors: Xiaoyang Jin; Lingyuan Meng; Zhao Yin; Haisheng Yu; Linnan Zhang; Weifeng Liang; Shouli Wang; Guanyuan Liu; Liguo Zhang
• Keywords: Cord blood

Abstract Dendritic cells (DCs) are professional antigen-presenting (APCs). The key functions of DCs include engulfing, processing and presenting antigens to T regulating the activation cells. There two major DC subtypes in human blood: plasmacytoid (pDCs) conventional DCs. To define differences between adult infant immune systems, especially terms constitution, we enriched from cord blood generated single-cell RNA sequencing data about 7000 using 10x Genomics Single Cell 3′ Solution. After incorporating differential expression analysis method our clustering process, identified all known dendritic cell subsets. Interestingly, also found a group with gene that was mix megakaryocytes pDCs. Further, verified selected genes at both level by PCR protein flow cytometry. This study further demonstrates power research.