Secondary metabolites from Lysobacter enzymogenes isolate LEC possess suppressive activity against microbial plant pathogens in vitro and in planta

• DOI: 10.1007/s42161-025-01896-0
• Publication Date: 2025-04-04T08:07:17Z
• Authors: Christian Drenker; Tam Dang; Doris El Mazouar; Sonja Weißhaupt; Agnes Mühlenweg; Lei Zhong; Roderich D. Süssmuth; Stefan Kunz; Annette Reineke; Yvonne Rondot; Ada Linkies; Eckhard Koch

Abstract In previous studies Lysobacter enzymogenes isolate LEC exhibited broad biocontrol activity against microbial phytopathogens on different crops. The aim of the present study was to identify metabolites responsible for antimicrobial and disease-suppressive activities this isolate. Fermentation broth fractionated by preparative HPLC resulting single fractions were analyzed HPLC-ESI-MS. vitro growth suppression apparent in agar diffusion microtiter plate assays Fusarium culmorum , Pythium ultimum Bacillus megaterium but not Escherichia coli . For F. Py. caused containing precursor ions m/z 511.28 ( z = 1) 513.30 1), that are characteristic members group polycyclic tetramate macrolactams (PoTeMs) like alteramides, heat stable antifungal factor (HSAF) derivatives thereof. inhibition B. a much broader spectrum also contained ions. tests with Phytophthora infestans climate chamber Pseudoperonospora cubensis cucumber plants carried out pooled groups from fractions. Significant dose-dependent exclusively observed PoTeMs, which agreement results assays. Activity comparable copper-containing fungicide concentration corresponded 3% or higher original fermentation broth. We therefore assume PoTeMs LEC. These findings confirm capability suitability as agent.