Evaluation of different crosslinking methods in altering the properties of extrusion-printed chitosan-based multi-material hydrogel composites

• DOI: 10.1007/s42242-022-00194-3
• Publication Date: 2022-04-01T15:13:10Z
• Authors: Suihong Liu; Haiguang Zhang; Tilman Ahlfeld; David Kilian; Yakui Liu; Michael Gelinsky; Qingxi Hu
• Keywords: Genipin; Gelatin; Biomaterial; Glutaraldehyde

Abstract Three-dimensional printing technologies exhibit tremendous potential in the advancing fields of tissue engineering and regenerative medicine due to precise spatial control over depositing biomaterial. Despite their widespread utilization numerous advantages, development suitable novel biomaterials for extrusion-based 3D scaffolds that support cell attachment, proliferation, vascularization remains a challenge. Multi-material composite hydrogels present incredible this field. Thus, work, multi-material hydrogel with promising formulation chitosan/gelatin functionalized egg white was developed, which provides good printability shape fidelity. In addition, series comparative analyses different crosslinking agents processes based on tripolyphosphate (TPP), genipin (GP), glutaraldehyde (GTA) were investigated compared select ideal strategy enhance physicochemical biological properties fabricated scaffolds. All results indicate resulting utilizing TPP have great engineering, especially supporting neo-vessel growth into scaffold promoting angiogenesis within engineered tissues. Graphic abstract