Isochorismate synthase is required for phylloquinone, but not salicylic acid biosynthesis in rice

• DOI: 10.1007/s42994-024-00166-4
• Publication Date: 2024-05-24T16:01:55Z
• Authors: Zengqian Wang; Guiqing Yang; Dandan Zhang; Guangxin Li; Jin-Long Qiu; Jie Wu
• Keywords: Phenylalanine ammonia-lyase

Abstract Salicylic acid (SA) is a phytohormone required for plant growth and defense signaling. There are two major SA biosynthesis pathways in plants: the isochorismate synthase (ICS) pathway phenylalanine ammonia-lyase (PAL) pathway. It has been demonstrated several species, including model Arabidopsis , that derived predominantly from ICS Here, we employed CRISPR/Cas9 system to generate knockout mutants rice ( Oryza sativa L.). The Osics display severe defects, completely devoid of phylloquinone, an isochorismate-derived product. defects can be rescued through exogenous application 1,4-dihydroxy-2-naphthoic (NA), precursor phylloquinone. Remarkably, basal levels not altered mutants. Our findings support role OsICS imply may occur alternative route other than