Plasma-Free Blood as a Potential Alternative to Whole Blood for Transcriptomic Analysis

• DOI: 10.1007/s43657-023-00121-1
• Publication Date: 2023-09-13T06:02:32Z
• Authors: Qingwang Chen; Xiaorou Guo; Haiyan Wang; Shanyue Sun; He Jiang; Peipei Zhang; Erfei Shang; Ruolan Zhang; Zehui Cao; Quanne Niu; Chao Zhang; Yaqing Liu; Leming Shi; Ying Yu; Wanwan Hou; Yuanting Zheng
• Keywords: 0303 health sciences; 03 medical and health sciences; Article

Abstract RNA sequencing (RNAseq) technology has become increasingly important in precision medicine and clinical diagnostics, emerged as a powerful tool for identifying protein-coding genes, performing differential gene analysis, inferring immune cell composition. Human peripheral blood samples are widely used RNAseq, providing valuable insights into individual biomolecular information. Blood can be classified whole (WB), plasma, serum, remaining sediment samples, including plasma-free (PFB) serum-free (SFB) that generally considered less useful byproducts during the processes of plasma serum separation, respectively. However, feasibility using PFB SFB transcriptome analysis remains unclear. In this study, we aimed to assess suitability employing or an alternative source transcriptomic analysis. We performed comparative WB, PFB, different applications. Our results revealed exhibit greater similarity WB than terms expression patterns, detection differentially expressed immunological characterizations, suggesting serve viable study contributes optimization sample utilization advancement research.