Inversion of the allosteric response of Escherichia coli glucosamine-6-P deaminase to N-acetylglucosamine 6-P, by single amino acid replacements

• DOI: 10.1016/j.abb.2003.09.041
• Publication Date: 2003-11-24T14:09:05Z
• Authors: David A. Cisneros; Gabriela M. Monte...; Samuel Lara-González; Mario L. Calcagno
• Keywords: Models, Molecular; 0301 basic medicine; Binding Sites; Recombinant Proteins; 3. Good health; Enzyme Activation; Isoenzymes; Kinetics; 03 medical and health sciences; Allosteric Regulation; Amino Acid Substitution; Escherichia coli; Mutagenesis, Site-Directed; Aldose-Ketose Isomerases; Allosteric Site

Amino acid replacements in the active site of glucosamine-6-P deaminase from Escherichia coli (GlcN6P deaminase, EC 3.5.99.6) involving the residues D141 and E148 produce atypical allosteric kinetics. These residues are located in the chain segment 139-156 which is part of the active site and which also forms several intersubunit contacts close to the allosteric site. In the D141N and E148Q mutant forms of this deaminase, there is an inversion of the effect of its physiological allosteric effector, N-acetylglucosamine 6-P, which becomes an inhibitor at substrate concentrations above a critical value. For both mutants, this particular point appears at low substrate concentration and the inhibition by the allosteric activator is the dominant effect in velocity versus substrate curves. These effects are analyzed as a particular case of the concerted allosteric model, assuming that the R state, the conformer displaying the higher affinity for the substrate, is the less catalytic state, thus producing an inverted allosteric response.