Denervation aggravates renal ischemia reperfusion injury via BMAL1-mediated Nrf2/ARE pathway

• DOI: 10.1016/j.abb.2023.109736
• Publication Date: 2023-08-30T15:11:03Z
• Authors: Chong Dong; Jing Li; Qiao Tang; Yifei Wang; Cheng Zeng; Li Du; Qian Sun
• Keywords: Renal ischemia

To explore the change of clock gene rhythm under renal denervation (RDN) and its effect on function oxidative stress during ischemia-reperfusion (IR) injury.C57/BL6 mice were randomly divided into 4 groups at daytime 7 A M (zeitgeber time [ZT] 0) or nighttime P (ZT12) in respectively: Sham (S) group, RDN IR group + (DIR) group. Renal pathological functional changes assessed by H&E staining, serum creatinine, urea nitrogen neutrophil gelatinase-associated lipocalin levels. was detected SOD MDA levels, inflammation measured IL-6, IL-17 F TNF-ɑ BMAL1, CLOCK, Nrf2 HO-1 mRNA protein expressions tested qPCR Western Blot.Compared with S groups, CLOCK genes kidney disordered while indexes did not significantly. Compared significantly higher DIR as well tissues. The nocturnal injury worst highest. In aggravated after Brusatol treatment, but there no significant improvement t-BHQ treatment night, which might be consistent expressions.RDN lead to disruption BMAL1-mediated accumulation kidney, reduced ability resist inflammation, due impaired activating Nrf2/ARE pathway nighttime.