Application of magnetic and core–shell nanoparticles to determine enrofloxacin and its metabolite using laser induced fluorescence microscope

• DOI: 10.1016/j.aca.2013.02.009
• Publication Date: 2013-02-18T18:14:51Z
• Authors: Suji Kim; Junga Ko; H.B. Lim
• Keywords: Enrofloxacin; Meat; Time Factors; Surface Properties; Lasers; 01 natural sciences; Anti-Bacterial Agents; 0104 chemical sciences; Immunoglobulin M; Microscopy, Fluorescence; Magnets; Humans; Nanoparticles; Antibodies, Immobilized; Fluoroquinolones

A unique analytical method using nanoparticles and laser-induced fluorescence microscopy (LIFM) was developed to determine enrofloxacin in this work. For sample pretreatment, two different kinds of particles, i.e., synthesized dye-doped core-shell silica nanoparticles and magnetic micro-particles (MPs), were used for fluorescent tagging and concentrating the enrofloxacin, respectively. The antibody of enrofloxacin was immobilized on the synthesized FITC-doped core-shell nanoparticles, and the enrofloxacin target was extracted by the MPs. At this moment, the average number of antibodies on each core-shell silica nanoparticle was ~0.9, which was determined by the fluorescence ratiometric method. The described method was demonstrated for a meat sample to determine enrofloxacin using LIFM, and the result was compared with enzyme-linked immunosorbent assay (ELISA). The developed technique allowed the simplified analytical procedure, improved the detection limit about 54-fold compared to ELISA.