Ion mobility spectrometry combined with ultra performance liquid chromatography/mass spectrometry for metabolic phenotyping of urine: Effects of column length, gradient duration and ion mobility spectrometry on metabolite detection

• DOI: 10.1016/j.aca.2017.06.020
• Publication Date: 2017-06-19T03:46:17Z
• Authors: Paul D. Rainville; Ian D. Wilson; Jeremy K. Nicholson; Giorgis Isaac; Lauren Mullin; James I. Langridge; Robert S. Plumb
• Keywords: Metabolome; Ion-mobility spectrometry

The need for rapid and efficient high throughput metabolic phenotyping (metabotyping) in metabolomic/metabonomic studies often requires compromises to be made between analytical speed metabolome coverage. Here the effect of column length (150, 75 30 mm) gradient duration (15, 7.5 3 min respectively) on number features detected when untargeted profiling human urine using reversed-phase ultra performance chromatography with, without, ion mobility spectrometry, has been examined. As would expected, reducing from 150 mm, duration, 15 min, resulted a reduction peak capacity 311 63 similar over ca. 16,000 6500. Under same chromatographic conditions employing UPLC/IMS/MS provide an additional orthogonal separation increase MS nearly 20,000 7500 mm columns respectively. Based this limited study potential LC/IMS/MS as tool improving increasing coverage clearly merits further depth study.