Scaffold-based tissue engineering: Supercritical carbon dioxide as an alternative method for decellularization and sterilization of dense materials

• DOI: 10.1016/j.actbio.2022.11.028
• Publication Date: 2022-11-22T01:33:31Z
• Authors: R.J.J. de Wit; D.J. van Dis; M.E. Bertrand; D. Tiemessen; S. Siddiqi; E. Oosterwijk; A.F.T.M. Verhagen
• Keywords: Decellularization; Sterilization; Supercritical Carbon Dioxide

Development of ready-to-use biomaterials and scaffolds is vital for further advancement scaffold-based tissue engineering in clinical practice. Scaffolds need to mimic 3D ultrastructure, have adequate mechanical strength, are biocompatible, non-immunogenic promote regeneration vivo. Although decellularization native tissues seems promising deliver that meet these criteria, hard, poorly penetrable diffusible remains challenging whilst being a very time-consuming process. In this study, method decellularize dense using supercritical carbon-dioxide preceded by freeze/thaw cycle followed several washing steps presented, demonstrating decellularisation efficiency substantially reduced production/handling time. Additionally, treatment was used as sterilization method, reducing the time required produce final scaffold. Histological evaluation showed that, after fine-tuning process, partially acellular scaffold obtained, with preservation glycosaminoglycans collagen fibers, albeit amount residual dsDNA still higher then chemically decellularized tissue. Biomechanical properties were similar native, non-decellularized After simulated functional outcome more trachea, when compared gamma irradiation. Thus, an effective cartilaginous materials, tuneable different demands other applications, but optimization may be required. STATEMENT OF SIGNIFICANCE: Further use engineered tracheal constructs restricted lack ideal Decellularized trachea considered scaffold, while forming consumable Decellularization carbon dioxide (scCO2) promising, resulting efficient removal cellular material production handling Addition scCO2 resulted reduction improving comparison traditional methods. This study presents alternative which can tuned applications.