Bone cell response to additively manufactured 3D micro-architectures with controlled Poisson's ratio: Auxetic vs. non-auxetic meta-biomaterials

• DOI: 10.1016/j.actbio.2024.01.045
• Publication Date: 2024-02-06T01:22:24Z
• Authors: Ebrahim Yarali; Maria Klimopoulou; Kristen David; Pouyan E. Boukany; Urs Staufer; Lidy E. Fratila-A...; Amir A. Zadpoor; Angelo Accardo; Mohammad J. Mirzaali
• Keywords: Auxetics; Poisson's ratio; Biomaterial; Aggregate modulus

The Poisson's ratio and elastic modulus are two parameters determining the behavior of biomaterials. While effects on cell response is widely studied, very little known regarding ratio. micro-architecture meta-biomaterials determines not only but also several other that influence response, such as porosity, pore size, effective modulus. It is, therefore, challenging to isolate from those micro-architectural parameters. Here, we computationally design with controlled ratios, ranging between -0.74 +0.74, while maintaining consistent 3D were additively manufactured at micro-scale using two-photon polymerization (2PP), mechanically evaluated meso‑scale. murine preosteoblasts these was then studied in vitro culture models. Meta-biomaterials positive ratios resulted higher metabolic activity than negative values. cells could attach infiltrate all bottom top, fully covering scaffolds after 17 days culture. Interestingly, exhibited different cell-induced deformations (e.g., shrinkage or local bending) observed via scanning electron microscopy. outcomes osteogenic differentiation (i.e., Runx2 immunofluorescent staining) matrix mineralization Alizarin red assays indicated significant potential impact field bone tissue engineering, paving way for development advanced meta-implants. STATEMENT OF SIGNIFICANCE: We meta-biomaterials. well-studied, ratio, especially values found architected biomaterials, remains largely unexplored. complexity arises intertwined parameters, porosity modulus, making it To overcome this limitation, study employed rational computational create alongside size. reveals polymerized displayed activity, developed supported well mineralization. pave models