Frequency of Nonallelic Homologous Recombination Is Correlated with Length of Homology: Evidence that Ectopic Synapsis Precedes Ectopic Crossing-Over

• DOI: 10.1016/j.ajhg.2011.09.009
• Publication Date: 2011-10-13T11:14:25Z
• Authors: Pengfei Liu; Melanie Lacaria; Feng Zhang; Marjorie Withers; P.J. Hastings; James R. Lupski
• Keywords: Recombination, Genetic; Comparative Genomic Hybridization; 0303 health sciences; Base Sequence; Models, Genetic; Molecular Sequence Data; Gene Dosage; Oligonucleotides; Chromosome Disorders; Cohort Studies; Chromosome Pairing; 03 medical and health sciences; Chromosome Duplication; Genetics; Trans-Activators; Humans; Genetics(clinical); Abnormalities, Multiple; Crossing Over, Genetic; Smith-Magenis Syndrome; Homologous Recombination; Alleles; Gene Deletion; Chromosomes, Human, Pair 17

Genomic disorders constitute a class of diseases that are associated with DNA rearrangements resulting from region-specific genome instability, that is, genome architecture incites genome instability. Nonallelic homologous recombination (NAHR) or crossing-over in meiosis between sequences that are not in allelic positions (i.e., paralogous sequences) can result in recurrent deletions or duplications causing genomic disorders. Previous studies of NAHR have focused on description of the phenomenon, but it remains unclear how NAHR occurs during meiosis and what factors determine its frequency. Here we assembled two patient cohorts with reciprocal genomic disorders; deletion associated Smith-Magenis syndrome and duplication associated Potocki-Lupski syndrome. By assessing the full spectrum of rearrangement types from the two cohorts, we find that complex rearrangements (those with more than one breakpoint) are more prevalent in copy-number gains (17.7%) than in copy-number losses (2.3%); an observation that supports a role for replicative mechanisms in complex rearrangement formation. Interestingly, for NAHR-mediated recurrent rearrangements, we show that crossover frequency is positively associated with the flanking low-copy repeat (LCR) length and inversely influenced by the inter-LCR distance. To explain this, we propose that the probability of ectopic chromosome synapsis increases with increased LCR length, and that ectopic synapsis is a necessary precursor to ectopic crossing-over.