Expression of miRNA-29a-3p and IFN-γ as biomarkers in active and latent pulmonary tuberculosis

• DOI: 10.1016/j.amsu.2022.104786
• Publication Date: 2022-09-29T04:10:20Z
• Authors: Nirmawati Angria; Muhammad Nasrum M...; Agussalim Bukhari; Irawaty Djaharuddin; Oslan Jumadi; Ahyar Ahmad; Upik Andriani Miskad; Rusdina Bte Ladju; Arif Santoso; Handayani Halik
• Keywords: 0301 basic medicine; 03 medical and health sciences; Case-controlled Study

Diagnosis and management of latent tuberculosis (TB) infections are one the challenges eradicating pulmonary TB. A critical aspect controlling TB spread is early diagnosis. One biological marker type under evaluation microRNAs (miRNAs). Mycobacterium infection causes epigenetic changes. The upregulation miRNA-29a-3p suppresses immune response by post-transcriptionally inhibiting interferon (INF)-γ expression in T cells, increasing susceptibility to This study aimed assess as a biomarker active infection.This case-control included 50 individuals with TB, 33 household contacts positive IFN-γ release assay (IGRA), 30 healthy controls. An enzyme-linked immunosorbent assay-based IGRA was used determine contacts. Quantitative real-time PCR quantify expression. Data analysis analyses variance receiver operating characteristic (ROC) curves.miRNA-29a-3p differed significantly between participants (controls; p = <0.001. ROC curve showed that had 86% sensitivity 73% specificity an area (AUC) 0.763 (95% confidence interval [CI]: 0.668-0.858). 84.8% 70% AUC 0.808 CI: 0.698-0.919) for Additionally, correlated (p < 0.0001) However, not INF-γ levels patients (R 0.005; 0.62) 0.010; 0.38) or controls 0.060; 0.19).miRNA-29a-3p increased Therefore, represents potential were miR-29a-3p