Olegusella massiliensis gen. nov., sp. nov., strain KHD7 T , a new bacterial genus isolated from the female genital tract of a patient with bacterial vaginosis

• DOI: 10.1016/j.anaerobe.2017.02.012
• Publication Date: 2017-02-21T07:15:41Z
• Authors: Khoudia Diop; Awa Diop; Florence Bretelle; Frédéric Cadoret; Caroline Michelle; Magali Richez; Jean-François Coc...; Didier Raoult; Pierre-Edouard Fo...; Florence Fenollar
• Keywords: Adult; DNA, Bacterial; Base Composition; Microscopy; 0303 health sciences; Fatty Acids; Sequence Analysis, DNA; Vaginosis, Bacterial; DNA, Ribosomal; Bacterial Typing Techniques; 3. Good health; Actinobacteria; 03 medical and health sciences; Cytosol; [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases; RNA, Ribosomal, 16S; Vagina; [SDV.MHEP.MI] Life Sciences [q-bio]/Human health and pathology/Infectious diseases; Cluster Analysis; Humans; Female; Anaerobiosis; Genome, Bacterial; Phylogeny

Strain KHD7T, a Gram-stain-positive rod-shaped, non-sporulating, strictly anaerobic bacterium, was isolated from the vaginal swab of a woman with bacterial vaginosis. We studied its phenotypic characteristics and sequenced its complete genome. The major fatty acids were C16:0 (44%), C18:2n6 (22%), and C18:1n9 (14%). The 1,806,744 bp long genome exhibited 49.24% G+C content; 1549 protein-coding and 51 RNA genes. Strain KHD7T exhibited a 93.5% 16S rRNA similarity with Olsenella uli, the phylogenetically closest species in the family Coriobacteriaceae. Therefore, strain KHD7T is sufficiently distinct to represent a new genus, for which we propose the name Olegusella massiliensis gen. nov., sp. nov. The type strain is KHD7T.