Effect of hydrated C60 fullerene on lipid, vitamin and amino acid composition in frozen-thawed ram semen

• DOI: 10.1016/j.anireprosci.2022.106939
• Publication Date: 2022-02-02T23:02:53Z
• Authors: Gaffari Türk; Recep H. Koca; İbrahim H. Güngör; Serap Dayan Cinkara; Tutku C. Acısu; Figen Erdem Erişir; Gözde Arkalı; Şeyma Özer Kaya; Meltem Kızıl; Mustafa Sönmez; Seyfettin Gür; Ökkeş Yılmaz; Abdurrauf Yüce; Mustafa Karatepe
• Keywords: Cryopreservation; Male; 0301 basic medicine; Sheep; Vitamins; Lipids; Spermatozoa; 03 medical and health sciences; Cryoprotective Agents; Semen; Sperm Motility; Animals; Fullerenes; Amino Acids; Semen Preservation

This study was conducted to investigate the effect of different doses of hydrated C60 fullerene (C60HyFn) on freeze-thawing process-induced changes in lipid, vitamin and amino acid composition and also in motility, kinematic, sperm quality and oxidative stress parameters in ram semen. Semen was collected from seven rams twice a week for 3 weeks, so six repetitions were performed. The semen collected in each repetition was pooled. Each pooled sample was diluted with tris + egg yolk extender with (200 nM, 400 nM, 800 nM, 1 µM and 5 µM) and without (control) C60HyFn and they were frozen in mini straws. The doses of 800 nM, 1 µM and 5 µM had higher total, progressive motility, sperm membrane functionality rates, glutathione-peroxidase and catalase activities. All doses of C60HyFn significantly reduced dead and total abnormal sperm rates and malondialdehyde levels. Significant increases in vitamin A (400 and 800 nM doses), vitamin K1 (400 nM, 800 nM and 1 µM doses), total amino acid (all doses) levels, but significant decreases in vitamin D2 (800 nM, 1 and 5 µM doses), vitamin D3 (1 and 5 µM doses) and vitamin E (200 nM, 1 and 5 µM) levels were observed compared to control. In conclusion, the addition of C60HyFn to ram semen at 200 nM - 5 µM range, especially at a dose of 800 nM, provides a positive contribution to the protection of motility, vitamins A, K and total amino acid levels, and oxidant/antioxidant balance after freeze-thawing.