Limonoids from seeds of Azadirachta indica A. Juss. and their cytotoxic activity

• DOI: 10.1016/j.apsb.2017.12.009
• Publication Date: 2018-02-01T14:46:47Z
• Authors: Jian Chen; Xiaona Fan; Jianhua Zhu; Liyan Song; Zhiwei Li; Fei Lin; Rongmin Yu; Hanhong Xu; Jiachen Zi
• Keywords: 0303 health sciences; 03 medical and health sciences; Original Article; Therapeutics. Pharmacology; RM1-950; 3. Good health

Four new limonoid-type nortriterpenoids, 1-detigloyl-1-O-methacryloylsalannin (1), 28-deoxo-2,3-dihydronimbolide (2), 12-acetoxy-3-O-acetyl-7-O-tigloylvilasinin (3) and 12-acetoxy-3-O-acetyl-7-O-methacryloylvilasinin (4), along with five known ones, were isolated from seeds of Azadirachta indica A. Juss. Their structures were elucidated by various spectroscopic methods, including UV, IR, MS, NMR, X-ray crystallography, quantum chemical calculation, as well as by comparison of their spectroscopic data with those reported. In the in vitro cytotoxic assay, 2 showed inhibitory activity against human breast cancer MDA-MB-231 cell line with IC50 value of 7.68±1.74 μmol/L, and 5 inhibited growth of human cervical cancer Hela cell line, melanoma A375 cell line and promyelocytic leukemia HL-60 cell line, with IC50 12.00±2.08, 17.44±2.11, and 13.95±5.74 μmol/L, respectively.