Transcriptomic analysis reveals the mechanism of low/high temperature resistance in an outstanding diet alga Nannochloropsis oceanica

• DOI: 10.1016/j.aqrep.2022.101365
• Publication Date: 2022-10-14T02:43:46Z
• Authors: Lin Zhang; Jiaojiao Tian; Lingzhi Ye; Kai Liao; Jichang Han; Song Wang; Jiayi Cao; Zhengwei Ye; Jilin Xu
• Keywords: 0301 basic medicine; 0303 health sciences; 03 medical and health sciences; Aquaculture. Fisheries. Angling; SH1-691; Nannochloropsis; Transcription factor; RNA-seq; Gene; Temperature resistance

Low/high temperature stress is one of the key abiotic factors restricting implementation microalgal large-scale production. Metabolism, involved in resistances, have been reported plants, but related research microalgae far behind. This study analyzed transcriptional changes Nannochloropsis oceanica under low and high stresses by RNA-seq technology. Results indicated that total differentially expressed gene (DEG) amount was much more than stress. GO enrichment analysis suggested DEGs were mainly concentrated protein modification phosphorus metabolic process, while transmembrane transport, lipid localization cellular homeostasis most enriched terms temperature. Moreover, "membrane" term both groups. The expressions 37 resistance-related enzymes/proteins (172 genes) concretely N. for first time. revealed ornithine decarboxylase (ODC) significantly down-regulated, choline dehydrogenase (CHDH), late embryogenesis abundant (LEA), dehydrin (DHN), chitinase (CHI), calmodulin (CaM), transfer (LTP) extremely up-regulated Under temperature, genes with drastic identified. Superoxide dismutase (SOD), ascorbate peroxidase (APX), glutathione synthetase (GSS), glyoxalase II (Gly II) exhibited down-regulated significantly, S-transferase (GST), CHDH, L-asparaginase (L-ASP), LEA, DHN, early response to dehydration (ERD), heat shock (HSP), CaM dramatically up-regulated. No significant detected (GPX), tocopherol cyclase (TC), gamma-glutamylcysteine (γ-GCS), pyrroline‐5–carboxylate reductase (P5CR), asparagine (ASN ), succinate-semialdehyde (SSADH), pyruvate (PDC), S-adenosylmethionine (SAMS) or Furthermore, 166 transcription factor (TF) plant resistance characterized categorized into 20 TF families. MYB active one, revealing family played a critical role oceanica. These new findings could benefit understanding mechanism Nannochloropsis. changed might be ideal candidates breeding elite strains stronger using genetic engineering methods.