Single nucleotide polymorphisms of the SIF gene and their association with growth traits in swimming crab (Portunus trituberculatus)

• DOI: 10.1016/j.aqrep.2023.101792
• Publication Date: 2023-10-23T14:24:29Z
• Authors: Baohua Duan; Tongxu Kang; Haifu Wan; Shumei Mu; Yueqiang Guan; Weibiao Liu; Zejian Li; Yang Tian; Yuqin Ren; Xianjiang Kang
• Keywords: Portunus trituberculatus; Hepatopancreas

The swimming crab (Portunus trituberculatus) is an economically important aquaculture species in China. Mining candidate single nucleotide polymorphisms (SNPs) and genes associated with growth traits of P. trituberculatus great importance for the genetic breeding this species. Among traits, "protein still life, isoforms C/SIF type 2 (SIF)" thought to be gene affecting animal development. In current study, we investigated SIF from better understand its biological function. full-length cDNA was 6185 bp, a 5208 bp open reading frame encoding 1735 amino acids. Homology analysis showed that shared 90.07% identity Procambarus clarkii 40.25–86.95% other Expression pattern fast- slow-growing groups revealed significantly higher expression level former than latter (P < 0.05). qPCR studies ubiquitous seven tissues tested, but most abundantly heart compared muscle, testis, ovary, seminal vesicle, gill, hepatopancreas A total 38 SNPs UTRs exons were screened genotyped 184 individuals crabs by targeted resequencing. Association four (full carapace width, length, body height, weight; P Two out shaped as haploblock containing three haplotypes (GC, TA, TC), TA haplotype GC haplotype. Overall, results provided potential SNP markers improvement breeding.