A new sex-specific genetic marker (fshr 1834G>T) for flathead grey mullet, Mugil cephalus, in Queensland, Australia

• DOI: 10.1016/j.aqrep.2023.101858
• Publication Date: 2023-12-01T18:03:34Z
• Authors: K.C. Anderson; J.A.T. Morgan; E.F. Goulden
• Keywords: Mugil; Flathead

In this study, genetic sex marker candidates from northern hemisphere Mugil cephalus were tested to see if they could be used a population originating south-east Queensland, Australia. As such, region of the follicle stimulating hormone receptor (fshr) gene was sequenced but did not contain previously published single nucleotide polymorphisms (SNPs). However, further screening fshr revealed promising candidate for Queensland M. cephalus, 1834 G>T, which accurate in 100% fish (excluding intersex fish, had female genotype). While all females homozygous G/G, males presented as either G/T (common) or T/T (lower frequency). Subsequently, real-time high-resolution melt developed facilitate rapid and genotyping based on G>T SNP. Initial results suggest that is useful SNP can reduce need more invasive sampling techniques such gonadal biopsy, provide information relating captive stock prior maturation, may prove wild surveys assessment.