Toxicity and detoxication assessment of juvenile black seabream (Acanthopagrus schlegelii) in response to dietary cadmium exposure: Based on growth performance and stress indicators

• DOI: 10.1016/j.aqrep.2023.101897
• Publication Date: 2023-12-23T23:15:36Z
• Authors: Xiaoyi Zhao; Wenli Zhao; Fangmin Xu; Yuedong Shen; Yangguang Bao; Bingqian Yang; Tingting Zhu; Xuemei Duan; Lefei Jiao; O.́scar Monroig; Qicun Zhou; Min Jin
• Keywords: XBP1; Malondialdehyde; ATF6

Cadmium (Cd) is a major pollutant in water, and long-term Cd exposure seriously threatens aquatic organisms. This study evaluated the effects of dietary on growth performance, tissue accumulation, oxidative stress, endoplasmic reticulum (ER) inflammation, apoptosis, anti-stress response juvenile black seabream (Acanthopagrus schlegelii). Six experimental diets containing different concentration were formulated: 0.02 (Control, Cd0.02), 1.60 (Cd1.60), 24.29 (Cd24.29), 49.04 (Cd49.04), 117.26 (Cd 117.26), 289.65 (Cd289.65) mg/kg. A feeding experiment was performed 540 seabreams (initial weight, 1.42 ± 0.01 g), which conducted for ten weeks. The findings revealed that diet lowered weight gain (WG) specific rate (SGR), but had no effect survival. Dramatically positive linear trends found between increasing contents including liver, kidney, muscle. Dietary increased malondialdehyde (MDA) production both serum hence, caused stress. stress-related genes, grp78, atf6, xbp1, markedly with levels, showing ER stress triggered. Likewise, transcriptional expression levels genes related to mt2, hsp70, hsp90 linearly up-regulated contents. Regarding pro-apoptosis caspase7, caspase9, bax anti-apoptosis gene bcl-2 all significantly promoted levels. Similar patterns linked inflammatory response, as increased, nuclear transcription factor nf-κb, pro-inflammatory cytokines tnfα il-1β, anti-inflammatory il-10 tgfβ dramatically elevated. In conclusion, supplementation suppressed deposition tissues, induced inflammation. contrast, juveniles also showed substantial tolerance by promoting ability.