Caloric restriction modifies spatiotemporal calcium dynamics in mouse hippocampal astrocytes

• DOI: 10.1016/j.bbamcr.2021.119034
• Publication Date: 2021-04-06T23:10:00Z
• Authors: Pavel Denisov; Alexander Popov; Alexey Brazhe; Natalia Lazareva; Alexei Verkhratsky; Alexey Semyanov
• Keywords: Male; Neurons; 0301 basic medicine; Brain; Gap Junctions; Hippocampus; Diet; Mice, Inbred C57BL; Mice; 03 medical and health sciences; Spatio-Temporal Analysis; Astrocytes; Animals; Calcium; Calcium Signaling; Neuroglia; Caloric Restriction

We analysed spatiotemporal properties of Ca2+ signals in protoplasmic astrocytes in the CA1 stratum radiatum of hippocampal slices from young (2-3 months old) mice housed in control conditions or exposed to a caloric restriction (CR) diet for one month. The astrocytic Ca2+ events became shorter in duration and smaller in size; they also demonstrated reduced velocity of expansion and shrinkage following CR. At the same time, Ca2+ signals in the astrocytes from the CR animals demonstrated higher amplitude and the faster rise and decay rates. These changes can be attributed to CR-induced morphological remodelling and uncoupling of astrocytes described in our previous study. CR-induced changes in the parameters of Ca2+ activity were partially reversed by inhibition of gap junctions/hemichannels with carbenoxolone (CBX). The effect of CBX on Ca2+ activity in CR-animals was unexpected because the diet already decreases gap junctional coupling in astrocytic syncytia. It may reflect the blockade of hemichannels also sensitive to this drug. Thus, CR-induced morphological remodelling of astrocytes is at least partly responsible for changes in the pattern of Ca2+ activity in the astrocytic network. How such changes in spatiotemporal Ca2+ landscape can translate into astrocytic physiology and neuron-glia interactions remains a matter for future studies.