115. “Inflammaging” and psychosocial stress in a murine orthotopic prostate cancer model

• DOI: 10.1016/j.bbi.2014.06.135
• Publication Date: 2014-08-22T14:52:20Z
• Authors: D.L. Bellinger; C. Molinaro; P. Gifford; M.S. Dulcich; R.E. Hartman; M.J. Pecaut; D. Lorton; D.S. Gridley
• Keywords: 03 medical and health sciences; 0302 clinical medicine; 3. Good health

Prostate cancer (PCa) is prevalent in older men, and the second leading cause of mortality. Aging and psychosocial stress affect tumor progression in other reproductive cancers, but interactions between age, stress and tumor progression in PCa remain unexplored. Since inflammation is a key player in tumor progression, we evaluated the effects of age and psychosocial stress on pro- and anti-inflammatory mediators in an orthotopic PCa model. Young (2 M) or old (18 M) male C57BL/6 mice with orthotopic RM-9 tumors were subjected to 14 days of combined social isolation and 2-h restraint (stressed) or remained group-housed in home cages (nonstressed). Twenty-five cytokines were quantified in tumor homogenates using multiplexed ELISAs. Age effects were evident for six proinflammatory cytokines ( p p