Targeting foamy macrophages by manipulating ABCA1 expression to facilitate lesion healing in the injured spinal cord

• DOI: 10.1016/j.bbi.2024.04.013
• Publication Date: 2024-04-16T23:58:07Z
• Authors: Xi Wang; Zhijian Cheng; Wenjiao Tai; Mingjun Shi; Maryam Ayazi; Yang Liu; Li Sun; Caiyong Yu; Zhongmin Fan; Bin Guo; Xijing He; Dongming Sun; Wise Young; Yi Ren
• Keywords: Male; Mice, Inbred C57BL; Mice, Knockout; Mice; Disease Models, Animal; Spinal Cord; Macrophages; Animals; Spinal Cord Injuries; ATP Binding Cassette Transporter 1; Foam Cells

Spinal cord injury (SCI) triggers a complex cascade of events, including myelin loss, neuronal damage, neuroinflammation, and the accumulation of damaged cells and debris at the injury site. Infiltrating bone marrow derived macrophages (BMDMϕ) migrate to the epicenter of the SCI lesion, where they engulf cell debris including abundant myelin debris to become pro-inflammatory foamy macrophages (foamy Mϕ), participate neuroinflammation, and facilitate the progression of SCI. This study aimed to elucidate the cellular and molecular mechanisms underlying the functional changes in foamy Mϕ and their potential implications for SCI. Contusion at T10 level of the spinal cord was induced using a New York University (NYU) impactor (5 g rod from a height of 6.25 mm) in male mice. ABCA1, an ATP-binding cassette transporter expressed by Mϕ, plays a crucial role in lipid efflux from foamy cells. We observed that foamy Mϕ lacking ABCA1 exhibited increased lipid accumulation and a higher presence of lipid-accumulated foamy Mϕ as well as elevated pro-inflammatory response in vitro and in injured spinal cord. We also found that both genetic and pharmacological enhancement of ABCA1 expression accelerated lipid efflux from foamy Mϕ, reduced lipid accumulation and inhibited the pro-inflammatory response of foamy Mϕ, and accelerated clearance of cell debris and necrotic cells, which resulted in functional recovery. Our study highlights the importance of understanding the pathologic role of foamy Mϕ in SCI progression and the potential of ABCA1 as a therapeutic target for modulating the inflammatory response, promoting lipid metabolism, and facilitating functional recovery in SCI.