L7/Pcp-2-specific expression of Cre recombinase using knock-in approach

• DOI: 10.1016/j.bbrc.2005.04.043
• Publication Date: 2005-04-21T11:40:57Z
• Authors: Hiromitsu Saito; Hideki Tsumura; Seiichi Otake; Akihiro Nishida; Takahisa Furukawa; Noboru Suzuki
• Keywords: Cre recombinase; Gene knockin; Conditional gene knockout; Gene knockout; Knockout mouse; Gene targeting

We report a knock-in mouse expressing Cre recombinase from the translational initiation site (ATG) of the endogenous L7/Pcp-2 gene. The resulting Cre expression matches the pattern of L7/Pcp-2 expression that is restricted to cerebellar Purkinje cells and retinal cells. Moreover, the Cre mouse showed no significant behavioral abnormality. Thus, our novel Cre mouse can be used for generation of Purkinje cells and retinal cell-specific gene expression and/or knockout in mouse using the Cre/loxP system.