Hsa-mir-182 suppresses lung tumorigenesis through down regulation of RGS17 expression in vitro

• DOI: 10.1016/j.bbrc.2010.04.127
• Publication Date: 2010-04-26T08:37:36Z
• Authors: Yihua Sun; Rong Fang; Chenguang Li; Li Li; Fei Li; Xiaolei Ye; Haiquan Chen
• Keywords: Ectopic expression

Lung cancer is one of the most devastating diseases worldwide. RGS17 is previously shown to be over-expressed in human lung adenocarcinomas and plays an important role in lung tumor growth. Here we have identified a miRNA, has-mir-182, involved in the regulation of RGS17 expression through two conserved sites located in its 3' UTR region. Consistently, endogenous RGS17 expression level is regulated by hsa-mir-182 in human lung cancer cell lines. Similar to the knockdown of RGS17, ectopic expression of hsa-mir-182 significantly inhibits lung cancer cell proliferation and anchorage-independent cell growth, which can be rescued by re-expression of RGS17. Taken together, these data have provided the first evidence of miRNA regulation of RGS17 expression in lung cancer.