Ginsenoside Rb1 promotes browning through regulation of PPARγ in 3T3-L1 adipocytes

• DOI: 10.1016/j.bbrc.2015.09.064
• Publication Date: 2015-09-14T14:08:59Z
• Authors: Qianqian Mu; Xin Fang; Xiaoke Li; Dandan Zhao; Fangfang Mo; Guangjian Jiang; Na Yu; Yi Zhang; Yubo Guo; Min Fu; Jun-Li Liu; Dongwei Zhang; Sihua Gao
• Keywords: 0301 basic medicine; Ginsenosides; Adipocytes, White; Cell Differentiation; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; Ion Channels; DNA-Binding Proteins; Mitochondrial Proteins; PPAR gamma; Mice; 03 medical and health sciences; Adipocytes, Brown; Glucose; Oxygen Consumption; 3T3-L1 Cells; Animals; Anilides; RNA, Messenger; Energy Metabolism; Uncoupling Protein 1; Transcription Factors

Browning of white adipocyte tissue (WAT) has received considerable attention due to its potential implication in preventing obesity and related comorbidities. Ginsenoside Rb1 is reported to improve glycolipid metabolism and reduce body weight in obese animals. However whether the body reducing effect mediates by browning effect remains unclear. For this purpose, 3T3-L1 adipocytes were used to study the effect of ginsenoside Rb1 on browning adipocytes specific genes and oxygen consumptions. The results demonstrate that 10 μM of ginsenoside Rb1 increases basal glucose uptake and promoted browning evidenced by significant increases in mRNA expressions of UCP-1, PGC-1α and PRDM16 in 3T3-L1 mature adipocytes. Further, ginsenoside Rb1 also increases PPARγ activity. And the browning effect is abrogated by GW9692, a PPARγ antagonist. In addition, ginsenoside Rb1 increases basal respiration rate, ATP production and uncoupling capacity in 3T3-L1 adipocytes. Those effects are also blunted by GW9692. The results suggest that ginsenoside Rb1 promote browning of 3T3-L1 adipocytes through induction of PPARγ. Our finding offer a new source to discover browning agonists and also useful to understand and extend the applications of ginseng and its constituents.