The pro-resolving lipid mediator Maresin 1 protects against cerebral ischemia/reperfusion injury by attenuating the pro-inflammatory response

• DOI: 10.1016/j.bbrc.2016.02.090
• Publication Date: 2016-02-24T12:34:27Z
• Authors: Wenjing Xian; Yan Wu; Wei Xiong; Longyan Li; Tong Li; Shangwen Pan; Limin Song; Lisha Hu; Lei Pei; Shanglong Yao; You Shang
• Keywords: Cerebral Cortex; Male; 0303 health sciences; Docosahexaenoic Acids; Neutrophils; Anti-Inflammatory Agents; NF-kappa B; Down-Regulation; Brain Ischemia; Mice, Inbred C57BL; Mice; 03 medical and health sciences; Reperfusion Injury; Nerve Degeneration; Animals; Cytokines; Inflammation Mediators; Neuroglia; Injections, Intraventricular

Inflammation plays a crucial role in acute ischemic stroke pathogenesis. Macrophage-derived Maresin 1 (MaR1) is a newly uncovered mediator with potent anti-inflammatory abilities. Here, we investigated the effect of MaR1 on acute inflammation and neuroprotection in a mouse brain ischemia reperfusion (I/R) model. Male C57 mice were subjected to 1-h middle cerebral artery occlusion (MCAO) and reperfusion. By the methods of 2,3,5-triphenyltetrazolium chloride, haematoxylin and eosin or Fluoro-Jade B staining, neurological deficits scoring, ELISA detection, immunofluorescence assay and western blot analysis, we found that intracerebroventricular injection of MaR1 significantly reduced the infarct volume and neurological defects, essentially protected the brain tissue and neurons from injury, alleviated pro-inflammatory reactions and NF-κB p65 activation and nuclear translocation. Taken together, our results suggest that MaR1 significantly protects against I/R injury probably by inhibiting pro-inflammatory reactions.