BATF2 balances the T cell-mediated immune response of CADM with an anti-MDA5 autoantibody

• DOI: 10.1016/j.bbrc.2021.02.128
• Publication Date: 2021-03-17T21:43:17Z
• Authors: Caiyan Li; Meidong Liu; Ke Liu; Muyuan Li; Yanjuan Liu; Tao Li; Yu Wei; Ying Long; Weijia He; Xueyan Shi; Yisha Li; Huali Zhang
• Keywords: CD4-Positive T-Lymphocytes; Male; 0301 basic medicine; Immunity, Cellular; Interferon-Induced Helicase, IFIH1; Tumor Suppressor Proteins; Cell Differentiation; Th1 Cells; T-Lymphocytes, Regulatory; Dermatomyositis; Up-Regulation; 3. Good health; 03 medical and health sciences; Basic-Leucine Zipper Transcription Factors; Humans; Th17 Cells; Female; RNA, Messenger; Autoantibodies

Clinically amyopathic dermatomyositis (CADM) is a subtype of dermatomyositis (DM) characterized by low-grade or absent muscle inflammation but frequent and rapidly progressive interstitial lung disease (RP-ILD) and skin ulcers with anti-melanoma differentiation-associated gene 5 (anti-MDA5) autoantibodies. Basic leucine zipper transcription factor ATF-like 2 (BATF2) is thought to function as an inhibitor of tumours and inflammation. Here, we aimed to investigate the roles of BATF2 in Th cell differentiation of CADM with an anti-MDA5 autoantibody (anti-MDA5+ CADM).Naive CD4+ T cells from human peripheral blood mononuclear cells (PBMCs) of healthy controls (HCs) were isolated and then cultured with IL-12, TGF-β or TGF-β plus IL-6 following anti-CD3 and anti-CD28 stimulations. The expression of BATF2 was measured by real-time PCR. The percentages of Th1, Th17 and Treg CD4+ T cells were detected by flow cytometry. BATF2 knockdown of CD4+ T cells was performed using small interfering RNAs (siRNAs).The expression of BATF2 in PBMCs was higher in anti-MDA5+ CADM patients than in healthy controls. The BATF2 mRNA expression was increased under Th1 and Treg polarization but decreased under Th17 polarization. Th17 cell activation-associated genes were possibly increased while Th1 and Treg cell differentiation-associated genes were inhibited by posttranscriptional gene silencing of BATF2 in CD4+ T cells.BATF2 promoted Th1 and Treg cell differentiation but suppressed Th17 cell activation in anti-MDA5+ CADM.