Growth hormone resistance induced by amino acid deprivation in fao cells is independent of FGF21

• DOI: 10.1016/j.bbrc.2024.149811
• Publication Date: 2024-03-23T00:27:02Z
• Authors: Maki Saito; Hiroki Nishi; Shin-Ichiro Takah...; Fumihiko Hakuno; Ichiro Miyata
• Keywords: FGF21

Adequate dietary intake of amino acids is imperative for normal animal growth. Our previous work using rat hepatocarcinoma Fao cells demonstrated that growth hormone (GH) resistance, coupled with a concurrent reduction in insulin-like factor 1 (Igf1) mRNA levels, may underlie the retardation associated low-protein diet (LPD). In this study, we investigated whether FGF21 contributes to liver GH resistance hepatoma under acid deprivation conditions. Mice subjected an LPD exhibited retardation, compromised signaling liver, and decreased blood IGF-1 levels compared those on control diet. To assess potential involvement fibroblast (FGF) 21, produced response deficiency, development examined Igf1 cultured acid-deprived medium. Despite inhibition Fgf21 expression by integrated stress inhibitor, inhibitor eukaryotic initiation 2-activating transcription 4 pathway, persisted deprivation. Additionally, introduction into medium did not impair either or GH-induced transcription. These data suggest that, cells, induces independently activity. By shedding light mechanisms behind retardation-associated linked deficiencies, our findings provide valuable insights clinicians formulating effective treatment strategies individuals facing these challenges.