Hepatocyte growth factor is necessary for efficient outgrowth of injured peripheral axons in in vitro culture system and in vivo nerve crush mouse model

• DOI: 10.1016/j.bbrep.2021.100973
• Publication Date: 2021-03-03T04:36:43Z
• Authors: Nayeon Lee; Sang Hwan Lee; Junghun Lee; Mi-Young Lee; Jaegook Lim; Subin Kim; Sunyoung Kim
• Keywords: Axotomy; Neurite; Peripheral nerve injury; Axoplasmic transport; Nerve Injury; Peripheral Nervous System

Hepatocyte growth factor (HGF) is a neurotrophic and its role in peripheral nerves has been relatively unknown. In this study, biological functions of HGF receptor c-met have investigated the context regeneration damaged nerves. Axotomy branch sensory neurons from embryonic dorsal root ganglia (DRG) resulted increased protein levels phosphorylated c-met. When neuronal cultures were treated with pharmacological inhibitor c-met, PHA665752, length axotomy-induced outgrowth neurite was significantly reduced. On other hand, addition recombinant proteins to culture facilitated axon outgrowth. nerve crush mouse model, level around injury site by almost 5.5-fold at 24 h post compared control mice maintained elevated for another 6 days. The amount sciatic also observed be higher than mice. PHA665752 locally applied nerve, mediated induction cJun effectively inhibited, indicating functional involvement HGF/c-met pathway process. extra exogenously provided intramuscular injection plasmid DNA expressing HGF, expression enhanced. Taken together, these results suggested that plays important roles directly interacting thus might useful tool developing therapeutics neuropathy.