Bioherbicide production by Diaporthe sp. isolated from the Brazilian Pampa biome

• DOI: 10.1016/j.bcab.2015.09.005
• Publication Date: 2015-09-14T18:15:54Z
• Authors: Angélica Rossana ...; Daiana Bortoluzzi...; Jessica Lima; Vitória Porto; Camila Marcuz; Rafael Camargo Fe...; Raquel C. Kuhn; Rodrigo J.S. Jacques; Jerson V.C. Guedes; Marcio A. Mazutti
• Keywords: 0106 biological sciences; 01 natural sciences

Abstract In this study was presented a rational strategy to scale-up a wild fungal strain from the Brazilian Pampa biome to produce compounds with herbicidal activity in pre-emergence. It was defined the medium composition for the growth and production of bioherbicide in shaken flasks using sucrose corn steep liquor as substrate. In a second step, the process variables in a stirred-tank bioreactor were assessed. Maximum biomass production in shaken flasks was 23.87 g L −1 (41% higher than synthetic media) and in the bioreator 27.8 g L −1 . The Diaporthe sp. growth in bioreactor was negatively affected by agitation higher than 40 rpm. The bioherbicide efficiency rate reached 100%, since none seed germinated after the application of fermented broth. The Brazilian Pampa biome could be an effective way to obtain high-value products from the biodiversity.