Enzymatic bromination of marine fungal extracts for enhancement of chemical diversity

• DOI: 10.1016/j.bcab.2023.102786
• Publication Date: 2023-07-16T12:02:43Z
• Authors: Bastien Cochereau; Thibaut Robiou du...; Yves François Pou...; Deniz Tasdemir; Laurence Meslet-C...; Catherine Roullier
• Keywords: Marine fungi; Aspergillus niger

This study reports for the first time use of a vanadium chloroperoxidase (vCPO) enzyme to increase chemical diversity marine fungal extracts generate new natural entities. The vCPO used is recombinant protein from fungus Hortaea werneckii (HwvCPO). It catalyzes formation hypohalous acid (HOX), highly reactive compound that can react with electron-rich substrates. Here, four obtained different strains (Penicillium expansum, Aspergillus pseudoglaucus, Trichoderma sp. and werneckii) were investigated enhancement their diversity. metabolomic showed enzymatic treatment P. expansum A. pseudoglaucus significantly boosted chemodiversity by increasing number halogenated molecules. Indeed, respectively 5.07 6.65 times more ions detected in ESI-MS profile compared negative controls. chemistry generated allowed identification brominated compounds, one which was further purified characterized as 12-bromo-communesin A (2). compound, contrast communesin (1), exhibited moderate antimicrobial activity on methillicin-resistant Staphylococcus aureus (IC50 62 μM). has clearly demonstrated employment be promising environmentally friendly strategy enhance extracts.